Journal: Journal of visualized experiments : JoVE
Article Title: Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia
doi: 10.3791/66093
Figure Lengend Snippet: Table of Materials
Article Snippet: Eur., USP (200 Proof) VWR 89125-174 Fine scissors - sharp Fine Science Tools 14061-10 Foundation B ™ Fetal Bovine Serum GeminiBio 900-208 Gilson ™ PIPETMAN ™ L Pipette Starter Kits FisherScientific F167370G Graefe Forceps Fine Science Tools 11051-10 Hank's Balanced Salt Solution (HBSS) 10X gibco 14185-052 Hemocytometer Fisher 02-671-10 Incubator BINDER C150-UL Kimwipes ® KIMTECH K222101 LABGARD Class II, Type A2 Biological Safety Cabinet Nuaire NU-425-400 LD Columns Miltenyi Biotec GmBH 130-042-901 LSE ™ Vortex Mixer CORNING 6775 LSRII/Fortessa/Symphony A1 Becton, Dickinson and Company 647800L6 MACS MULTI STAND Miltenyi Biotec GmBH 130-042-303 MACsmix ™ Tube Rotator Miltenyi Biotec GmBH 130-090-753 mIgG Millipore-Sigma 18765-10mg 2 mg/mL Nup98-HoxD13 (NHD13) Mice Jackson Laboratory 010505 PE anti-mouse CD51 Antibody (RMV-7) Biolegend 104106 0.2 mg/mL PE/Cyanine5 anti-mouse CD140a Antibody (RUO) Biolegend 135920 0.2 mg/mL Penicillin-Streptomycin Gibco 15140122 10,000 U/mL Plastipak ™ 3 mL Syringe Becton, Dickinson and Company 309657 Propidium Iodide - 1.0 mg/mL Solution in Water ThermoFisher Scientific P3566 QuadroMACS Separator Miltenyi Biotec GmBH 130-090-976 Sorvall X Pro / ST Plus Series Centrifuge Thermo Scientific 75009521 TER-119 Monoclonal Antibody (TER-119), APC Invitrogen 17-5921-82 0.2 mg/mL Trypan Blue Solution 0.4% Gibco 15-250-061 Ultrapure 0.5 M EDTA, pH 8.0 Invitrogen 15575-038 Open in a separate window Table of Materials.
Techniques: Transferring, Sterility, Suction Filtration, Evaporation, Purification, Blocking Assay, Lysis
